2PWF
Crystal structure of the MutB D200A mutant in complex with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9330 |
Spacegroup name | P 1 |
Unit cell lengths | 63.980, 85.810, 122.140 |
Unit cell angles | 81.77, 81.43, 89.94 |
Refinement procedure
Resolution | 45.850 - 1.800 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.23800 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 225077 | |
<I/σ(I)> | 8.86 | 3.08 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 13%PEG20000, 0.1M Hepes, 0.01M L-cysteine, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |