2PWE
Crystal structure of the MutB E254Q mutant in complex with the substrate sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 |
Unit cell lengths | 64.310, 73.730, 82.750 |
Unit cell angles | 66.18, 74.51, 72.55 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.190 |
R-free | 0.23500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 85358 | |
<I/σ(I)> | 12.38 | 4.29 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 11% PEG20000, 0.1M Hepes, 0.01M L-cysteine, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |