2PW9
Crystal structure of a putative formate dehydrogenase accessory protein from Desulfotalea psychrophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.987, 99.257, 88.502 |
Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.651 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.375 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.108 | 0.434 |
Number of reflections | 57870 | |
<I/σ(I)> | 12.4 | 2.6 |
Completeness [%] | 98.2 | 97.4 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 294 | 100 mM Sodium MES pH 6.5, 22% PEG 8000, 200 mM Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |