2PW9
Crystal structure of a putative formate dehydrogenase accessory protein from Desulfotalea psychrophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.987, 99.257, 88.502 |
| Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.651 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.375 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.108 | 0.434 |
| Number of reflections | 57870 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100 mM Sodium MES pH 6.5, 22% PEG 8000, 200 mM Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
