2PW5
Crystal Structure of Staphylococcal nuclease variant V66Y/P117G/H124L/S128A at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 296 |
Detector technology | CCD |
Collection date | 2006-05-24 |
Detector | APEX II CCD |
Spacegroup name | P 41 |
Unit cell lengths | 49.022, 49.022, 63.838 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.192 |
Rwork | 0.182 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Staphylococcal nuclease V66E/P117G/H124L/S128A variant (at 100K) with b-factors set to 20.0 A^2 and residue 66 truncated to Ala |
Data reduction software | APEX |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.033 | 0.222 |
Number of reflections | 8913 | |
<I/σ(I)> | 24.29 | 4.28 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.86 | 5.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 38% MPD, 0.025M potassium phosphate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |