2PVU
Crystal structures of the arginine-, lysine-, histidine-binding protein ArtJ from the thermophilic bacterium Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95373 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.268, 73.887, 102.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.370 - 1.790 |
R-factor | 0.17689 |
Rwork | 0.174 |
R-free | 0.22385 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.563 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.085 | 0.351 |
Number of reflections | 21953 | |
<I/σ(I)> | 23.49 | 3.5 |
Completeness [%] | 93.1 | 66.4 |
Redundancy | 6.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M ammoniumsulfate, 30.5 % w/v polyethylene glycol (PEG) 2000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |