2PVT

Crystal structure of a new isoform of phospholipase A2 from russells viper at 2.1 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2007-01-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54132
Spacegroup name	P 43
Unit cell lengths	53.530, 53.530, 48.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.940 - 2.100
R-factor	0.16662
Rwork	0.166
R-free	0.21735
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sv3
RMSD bond length	0.018
RMSD bond angle	1.825
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.450	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	7442
Completeness [%]	96.2	80.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	0.3M Ammonium sulphate, 30% PEG8000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K