2PVR
Crystal structure of the catalytic subunit of protein kinase CK2 (C-terminal deletion mutant 1-335) in complex with two sulfate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.670, 45.371, 63.442 |
| Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
| Resolution | 19.710 - 1.605 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.24146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pjk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.710 | 1.700 |
| High resolution limit [Å] | 1.605 | 1.605 |
| Number of reflections | 40464 | |
| <I/σ(I)> | 10.5 | 3.2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 4.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEGmme 5000, ammonium sulfate, MES, adenylyl imidodiphosphate, magnesium chloride, peptide RRRADDSDDDDD, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
