2PVI

PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	289
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	95.800, 86.300, 48.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 1.760
R-factor	0.188
Rwork	0.188
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pvi
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	1.840
High resolution limit [Å]	1.760	1.760
Rmerge	0.054
<I/σ(I)>	14.1
Completeness [%]	77.3	23.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5		pH 4.5, VAPOR DIFFUSION, HANGING DROP