2PVB
PIKE PARVALBUMIN (PI 4.10) AT LOW TEMPERATURE (100K) AND ATOMIC RESOLUTION (0.91 A).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.030, 49.810, 34.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 0.910 |
| R-factor | 0.1095 |
| R-free | 0.13200 * |
| Structure solution method | OTHER |
| Starting model (for MR) | 1pvb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 0.920 |
| High resolution limit [Å] | 0.910 | 0.910 |
| Rmerge | 0.036 * | |
| Total number of observations | 481972 * | |
| Number of reflections | 63698 | |
| <I/σ(I)> | 14.2 | 3.3 |
| Completeness [%] | 99.8 | 97.3 |
| Redundancy | 4.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 | under microgravity, Declercq, J.P., (1999) J.Cryst.Growth, 196, 595. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium azide | 0.02 (%) | |
| 2 | 1 | drop | protein | 25-35 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.25-2.40 (M) | |
| 4 | 1 | reservoir | EDTA | 0.3 (mg/ml) | |
| 5 | 1 | reservoir | Tris-HCl |
