2PVA
OXIDIZED PENICILLIN V ACYLASE FROM B. SPHAERICUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 208.400, 208.400, 96.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.196 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.082 |
| Total number of observations | 239296 * |
| Number of reflections | 80217 |
| <I/σ(I)> | 14 |
| Completeness [%] | 97.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 5MM DTT, 30% AS, 1% SUCROSE, 0.2M NA PHOSPHATE, PH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | reservoir | sodium phosphate | 0.2 (M) | pH6.4 |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | ||
| 6 | 1 | reservoir | sucrose | 10 (%(w/v)) |
