2PV0
DNA methyltransferase 3 like protein (DNMT3L)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0,1.2199,1.2815,1.2826,1.2830 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 267.200, 267.200, 149.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.740 - 3.300 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.410 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.095 | 0.489 |
| Number of reflections | 47346 | |
| <I/σ(I)> | 13.4 | 3.9 |
| Completeness [%] | 98.9 | 99.1 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | 0.4-0.6 M sodium citrate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | 0.4-0.6 M sodium citrate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
