2PUY
Crystal Structure of the BHC80 PHD finger
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2006-02-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97917, 1.28295, 1.28341 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.511, 25.386, 62.282 |
Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.430 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.470 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.089 | 0.237 |
Number of reflections | 23287 | |
<I/σ(I)> | 16 | 8.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% Isopropanol, 5-10% polyethylene glycol 4000, 100mM MES 6.2-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |