2PUK
Crystal structure of the binary complex between ferredoxin: thioredoxin reductase and thioredoxin m
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.952, 42.223, 145.344 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 145.865 | 72.740 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.100 | 0.069 | 0.291 |
| Total number of observations | 1337 | 6678 | |
| Number of reflections | 13477 | ||
| <I/σ(I)> | 5.5 | 6.1 | 2.5 |
| Completeness [%] | 99.8 | 95.6 | 99.7 |
| Redundancy | 3.6 | 2.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
