2PUK
Crystal structure of the binary complex between ferredoxin: thioredoxin reductase and thioredoxin m
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 53.952, 42.223, 145.344 |
| 格子定数 [度] | 90.00, 90.29, 90.00 |
精密化法
| 分解能 | 30.000 - 3.000 |
| R因子 | 0.237 |
| Rwork | 0.235 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 145.865 | 72.740 | 3.160 |
| 分解能 [Å] (高) | 3.000 | 9.490 | 3.000 |
| Rmerge_l_obs | 0.100 | 0.069 | 0.291 |
| Total number of observations | 1337 | 6678 | |
| 独立反射数 | 13477 | ||
| <I/σ(I)> | 5.5 | 6.1 | 2.5 |
| 完全性 [%] | 99.8 | 95.6 | 99.7 |
| 冗長性 | 3.6 | 2.9 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
