2PTV
Structure of NK cell receptor ligand CD48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 78.870, 78.870, 31.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.660 |
| R-factor | 0.20487 |
| Rwork | 0.202 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ptt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.761 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.047 | 0.200 |
| Number of reflections | 11309 | |
| <I/σ(I)> | 66.7 | 8.4 |
| Completeness [%] | 97.3 | 81.5 |
| Redundancy | 12.9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 30% (w/v) PEG 8000, 100 mM sodium imidazole, 0.2 M NaCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
