2PTT
Structure of NK cell receptor 2B4 (CD244) bound to its ligand CD48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.938, 37.270, 94.740 |
| Unit cell angles | 90.00, 108.68, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.630 |
| R-factor | 0.19279 |
| Rwork | 0.190 |
| R-free | 0.24365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hng |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.055 | 0.180 |
| Number of reflections | 28061 | |
| <I/σ(I)> | 53.6 | 8 |
| Completeness [%] | 99.1 | 97.4 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6 M ammonium sulfate, 0.1 mM MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
