2PTM
Structure and rearrangements in the carboxy-terminal region of SpIH channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | CCD |
| Collection date | 2004-04-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 92.354, 92.354, 63.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.205 - 1.930 |
| Rwork | 0.187 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 21201 | |
| <I/σ(I)> | 30.5 | 7.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 13.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 10 % w/v PEG 6000, 0.5 M NaCl, 20 % glycerol, 0.1 M HEPES, 10 mM hexamine cobalt trichloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
