2PTK
CHICKEN SRC TYROSINE KINASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BL19 |
| Synchrotron site | ESRF |
| Beamline | BL19 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.561, 89.252, 97.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.350 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.038 * | 0.218 * |
| Number of reflections | 19579 | |
| <I/σ(I)> | 22 | 4.3 |
| Completeness [%] | 99.7 | 99.6 * |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.2 | 16% PEG-2000 10% PEG-400 100MM TRIS PH=8.2 300MM NACL 1MM EDTA 1MM DTT 1MM AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG2000 | 16-18 (%) | |
| 2 | 1 | 1 | PEG400 | 10 (%) | |
| 3 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 4 | 1 | 1 | 300 (mM) | ||
| 5 | 1 | 1 | EDTA | 1 (mM) | |
| 6 | 1 | 1 | sodium azide | 1 (mM) | |
| 7 | 1 | 1 | reduced dithiothreitol | 1 (mM) |
