2PTF
Crystal structure of protein MTH_863 from Methanobacterium thermoautotrophicum bound to FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.727, 91.727, 117.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.568 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.883 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.087 | 0.413 |
| Number of reflections | 21594 | |
| <I/σ(I)> | 26 | 5.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.5 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 100 mM Tris-HCl, 50% MPD, 100 mM Ammonium phosphate, pH 8.5, VAPOR DIFFUSION, temperature 294K |
