2PT7
Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97590 |
Spacegroup name | P 1 |
Unit cell lengths | 86.851, 86.900, 104.129 |
Unit cell angles | 111.04, 95.98, 104.94 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.23185 |
Rwork | 0.230 |
R-free | 0.26966 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g6o |
RMSD bond length | 0.012 |
RMSD bond angle | 1.197 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.916 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.091 | 0.243 |
Number of reflections | 100842 | |
<I/σ(I)> | 5 | 2.9 |
Completeness [%] | 96.3 | 96.5 |
Redundancy | 3.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM Mes, 8% polyethylene glycol 8,000, 10mM ethylene-diamine-tetra-acetic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |