2PT1
FutA1 Synechocystis PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.170, 60.680, 91.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Y9U Bordetella ferric binding protein |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.108 |
| Data reduction software | SAINT |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 21638 | |
| <I/σ(I)> | 19.6 | 5.1 |
| Completeness [%] | 96.1 | 81.3 |
| Redundancy | 3.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch seeding | 5 | 298 | 100mM succinate, 2.2-2.8 ammonium sulfate, 5% iso-propanol, pH 5.0, batch seeding, temperature 298K |
