2PSZ
Structure of the PTP-like Phytase expressed by Selenomonas ruminantium at low ionic strength
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.917, 137.698, 80.096 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.183 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2B4P |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.228 |
| Number of reflections | 61360 | |
| <I/σ(I)> | 15.6 | 2.97 |
| Completeness [%] | 93.8 | 63.2 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 10% P8K, 200 mM NaCl, 50 mM Na-acetate (pH 4.8), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
