2PST
1.8A Crystal Structure of the PA2412 protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2005-07-30 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 |
Unit cell lengths | 51.543, 51.543, 32.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.500 - 1.800 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.265 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.072 | 0.285 |
Number of reflections | 7822 | |
<I/σ(I)> | 13.9 | 2.3 |
Completeness [%] | 96.3 | 72.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5 | 277 | Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K |
1 | MICROBATCH | 5 | 277 | Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K |
1 | MICROBATCH | 5 | 277 | Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K |
1 | MICROBATCH | 5 | 277 | Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K |