2PSP
Porcine pancreatic spasmolytic polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.600, 60.600, 113.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.25800 |
RMSD bond length | 0.014 |
RMSD bond angle | 26.760 * |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.950 |
Rmerge | 0.058 |
Number of reflections | 15652 |
Completeness [%] | 87.3 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | 2.0 (M) | ||
3 | 1 | reservoir | PEG400 | 7 (%(w/v)) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |