2PSO
Human StarD13 (DLC2) lipid transfer and protein localization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03992 |
| Spacegroup name | P 43 |
| Unit cell lengths | 78.240, 78.240, 212.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.800 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jss |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 31213 | |
| <I/σ(I)> | 16.69 | 3.52 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20 % MPD, 100 mM Tris-HCl, 125 mM NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
