2PSM
Crystal structure of Interleukin 15 in complex with Interleukin 15 receptor alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 118.450, 118.450, 76.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.190 |
Rwork | 0.224 |
R-free | 0.23600 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 53064 | |
<I/σ(I)> | 23 | 18.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 1.7M Na/K Phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |