2PSF

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Collection date	2005-09-17
Spacegroup name	P 21 21 21
Unit cell lengths	81.178, 82.279, 90.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.400
R-factor	0.17366
Rwork	0.173
R-free	0.19406
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.209
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.400
Number of reflections	111557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.4M sodium acetate, 20% w/v PEG 3350, 1.8mM n-Decyl-beta-D-thiomaltoside, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K