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2PSD

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL11-1
Synchrotron siteSSRL
BeamlineBL11-1
Temperature [K]100
Spacegroup nameP 61
Unit cell lengths119.468, 119.468, 47.995
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution50.000 - 1.400
R-factor0.16631
Rwork0.165
R-free0.18341
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.372
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]1.400
Number of reflections73121
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82930.3M NaCl, 1.25M diammonium phosphate, 0.1M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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PDB entries from 2024-11-20

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