2PSB
Crystal structure of YerB protein from Bacillus subtilis. NorthEast Structural Genomics target SR586
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 32 |
| Unit cell lengths | 43.216, 43.216, 128.835 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.220 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.525 |
| Number of reflections | 29886 | |
| <I/σ(I)> | 12.9 | 10.2 |
| Completeness [%] | 96.0 | 73 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 8.5 | 277 | 1M Tris-HCl, PEG 10000, 1,4-Butanediol, pH 8.5, MICROBATCH UNDER OIL, temperature 277K |
