2PRV

Crystal structure of an uncharacterized protein (yobk, bsu18990) from bacillus subtilis at 1.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97928, 0.97895
Spacegroup name	P 1
Unit cell lengths	40.128, 45.200, 48.083
Unit cell angles	84.54, 67.52, 73.12

Refinement procedure

Resolution	24.268 - 1.300
R-factor	0.137
Rwork	0.135
R-free	0.17500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.660
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.268	24.390	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.055	0.045	0.524
Total number of observations		2897	19923
Number of reflections	69476
<I/σ(I)>	7.2	13.7	1.4
Completeness [%]	94.5	92.9	92.3
Redundancy	3.9	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	NANODROP, 2.5M Ammonium sulfate, 0.15M Potassium sodium tartrate, 0.1M Citric acid pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K