2PRK
SYNCHROTRON X-RAY DATA COLLECTION AND RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.170, 68.170, 108.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 * - 1.500 |
| R-factor | 0.167 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Rmerge | 0.081 * |
| Total number of observations | 36039 * |
| Number of reflections | 10694 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | 2-amino-2-(hydroxymethyl)-1,3-propanediol |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | 1 (M) | ||
| 5 | 1 | reservoir | 0.02 (%) |
