Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Collection date | 1994-08-09 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.500, 128.500, 116.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.170 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.23700 |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 3.600 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.170 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | |
| Total number of observations | 62732 * | |
| Number of reflections | 35730 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 84.0 * | 46.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 16 * | GLYCOGEN PHOSPHORYLASE WAS CO-CRYSTALLISED WITH 20 MM N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE IN A MEDIUM CONSISTING OF 20-30 MG/ML ENZYME, 1 MM IMP, 1 MM SPERMINE, 10 MM BES, 3 MM DITHIOTHREITOL, 0.1 MM EDTA, 0.02% (W/V) SODIUM AZIDE, PH 6.7 AT 16 C, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | BES | ||
| 3 | 1 | 1 | DITHIOTHREITOL | ||
| 4 | 1 | 1 | NAN3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1-GlcNAc | 20 (mM) | in a medium consisting | |
| 2 | 1 | enzyme | 20-30 (mg/ml) | ||
| 3 | 1 | IMP | 1 (mM) | ||
| 4 | 1 | spermine | 1 (mM) | ||
| 5 | 1 | 1 | BES | 10 (mM) | |
| 6 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 7 | 1 | 1 | EDTA | 0.1 (mM) | |
| 8 | 1 | 1 | sodium azide | 0.02 (%(w/v)) |
