2PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (UBIQUINONE-2 COMPLEX)

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1992-09-25
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.450
R-factor	0.171 *
Rwork	0.182
R-free	0.22000 *
Structure solution method	DIFFERENCE FOURIER, SA OMIT MAPS
Starting model (for MR)	1prc
RMSD bond length	0.012
RMSD bond angle	23.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.700	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.096	0.261
Total number of observations	220257 *
Number of reflections	79397 *
<I/σ(I)>	5.7	2.3
Completeness [%]	76.5	54.8 *
Redundancy	2.8	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.5 (M)
2	1	reservoir	ammonium sulfate	3 (M)	or 2.6M or 2.4M