2PRB
crystal structure of aminoglycoside acetyltransferase AAC(6')-Ib in complex whith coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9310 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.620, 57.620, 146.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.760 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pr8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.630 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.183 |
| Total number of observations | 35626 | |
| Number of reflections | 23836 | |
| <I/σ(I)> | 8.3 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.5 M K2HPO4 0.06M NaH2PO4 0.1M guanidine-HCl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
