2PR8

crystal structure of aminoglycoside N-acetyltransferase AAC(6')-Ib11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	77
Detector technology	CCD
Collection date	2005-09-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9340
Spacegroup name	C 2 2 21
Unit cell lengths	71.620, 85.370, 150.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.100 - 2.100
R-factor	0.201
Rwork	0.196
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.687
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.100	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.045	0.190
Number of reflections	26857
<I/σ(I)>	20.42	7.3
Completeness [%]	98.2	97.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K