2PR7

Crystal structure of uncharacterized protein (NP_599989.1) from Corynebacterium glutamicum ATCC 13032 Kitasato at 1.44 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97929
Spacegroup name	P 1
Unit cell lengths	29.149, 45.153, 60.777
Unit cell angles	100.72, 103.86, 103.31

Refinement procedure

Resolution	39.193 - 1.440
R-factor	0.17
Rwork	0.168
R-free	0.19900
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.543
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.193		1.490
High resolution limit [Å]	1.440	2.460	1.440
Rmerge	0.036	0.032	0.467
Number of reflections	40271
<I/σ(I)>	14.96	26.2	2.2
Completeness [%]	77.4	95.9	27.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	NANODROP, 10.0% 2-propanol, 0.3M Calcium acetate, 0.1M MES pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K