2PR6

Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Light Structure)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	90.295, 91.678, 34.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.850 - 1.950
R-factor	0.184
Rwork	0.181
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	1.980
High resolution limit [Å]	1.950	5.220	1.950
Rmerge	0.086	0.047	0.371
Number of reflections	21669
<I/σ(I)>	7.8
Completeness [%]	99.9	99.6	99.8
Redundancy	7.5	6.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M Na acetate pH 4.6, 18-22 % (w/v) PEG-4000, 75-150 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K