2PR0
Crystal structure of Sylvaticin, a new secreted protein from Pythium Sylvaticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.480, 1.239 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.692, 25.974, 68.349 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 25.900 - 1.720 |
| R-factor | 0.204 |
| Rwork | 0.194 |
| R-free | 0.18570 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.048 | 0.073 |
| Number of reflections | 18321 | |
| <I/σ(I)> | 24.2 | 12.5 |
| Completeness [%] | 92.9 | 71.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 2000 MME, 0.01M NiCl2, 0.1M Tris-HKL pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
