2PQX
E. coli RNase 1 (in vivo folded)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.894, 49.626, 53.398 |
| Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.420 |
| Rwork | 0.188 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.554 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 39060 | |
| <I/σ(I)> | 15.75 | 3.95 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.1 M MES pH 6.1, 0.1 M NH4AC, 10 % glycerol, 1 mM MgCl2, and 25 % polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
