2PQW
Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 17-25), trigonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.013, 117.013, 90.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.790 - 2.000 |
| R-factor | 0.19661 |
| Rwork | 0.195 |
| R-free | 0.22804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oz2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.415 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.790 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.718 |
| Number of reflections | 48125 | |
| <I/σ(I)> | 12.5 | 1.4 |
| Completeness [%] | 99.1 | 96.9 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 0.1M Sodium acetate pH 4.6, 4% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
