2PQJ
Crystal structure of active ribosome inactivating protein from maize (b-32), complex with adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 |
| Unit cell lengths | 115.908, 115.908, 44.898 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.990 - 2.800 |
| R-factor | 0.2319 |
| Rwork | 0.232 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pqi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.415 |
| Number of reflections | 16621 | |
| <I/σ(I)> | 6.4 | 1.8 |
| Completeness [%] | 98.9 | 92.8 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Sodium acetate tri-hydrate, 0.1 M Tris-HCl pH 8.5, 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
