2PQ3
N-Terminal Calmodulin Zn-Trapped Intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-29 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 35.211, 35.211, 143.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.300 |
| R-factor | 0.1431 |
| Rwork | 0.143 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 12687 | |
| <I/σ(I)> | 46.1 | 3.8 |
| Completeness [%] | 99.0 | 92.1 |
| Redundancy | 12.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 100mM Na Cacodylate, 5mM Zn Acetate, 16% PEG8000, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
