2PQ2
Structure of serine proteinase K complex with a highly flexible hydrophobic peptide at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.314, 68.314, 108.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.300 - 1.820 |
| R-factor | 0.17016 |
| Rwork | 0.168 |
| R-free | 0.20728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dp4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.224 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.300 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 23699 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 99.6 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.2M AMMONIUM ACTATE, 0.1M SODIUM ACETATE, 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
