2PQ0
Crystal structure of Hyopthetical protein (gk_1056) from geobacillus Kaustophilus HTA426
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-02 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97882, 0.97938, 0.90000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.050, 114.050, 182.309 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.070 - 2.600 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.066 | 0.356 |
Number of reflections | 67421 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 95mM NaCl, 95mM Hepes, 1.52M Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |