2PPV
CRYSTAL STRUCTURE OF a protein belonging to the UPF0052 (SE_0549) FROM STAPHYLOCOCCUS EPIDERMIDIS ATCC 12228 AT 2.00 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97931, 0.91837 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.666, 136.814, 55.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.185 - 2.000 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.480 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.185 | 29.180 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.101 | 0.040 | 0.870 |
| Total number of observations | 1469 | 10662 | |
| Number of reflections | 35353 | ||
| <I/σ(I)> | 6.2 | 16 | 0.8 |
| Completeness [%] | 98.8 | 89.1 | 99.7 |
| Redundancy | 4.1 | 3.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | NANODROP, 0.2M Di-ammonium hydrogen phosphate, 25.0% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
