2PPT
Crystal structure of thioredoxin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-06 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.784, 68.184, 64.124 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.066 |
| Number of reflections | 24706 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 96.2 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 28-32% PEG400, 10 mM zinc sulfate, 50 mM ADA , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 6.50 |
