2PPS
PHOTOSYNTHETIC REACTION CENTER AND CORE ANTENNA SYSTEM (TRIMERIC), ALPHA CARBON ONLY
Replaces: 1PPSExperimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 286.000, 286.000, 167.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 4.000 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 3.950 |
| High resolution limit [Å] | 3.850 | 3.850 |
| Rmerge | 0.085 | 0.173 * |
| Number of reflections | 69153 | |
| <I/σ(I)> | 6.4 | 3.6 |
| Completeness [%] | 94.4 | 81.3 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.4 | 4 * | PROTEIN CONCENTRATION:80 MG/ML METHOD: MICRODIALYSIS BUFFER: 0.02% BETA-DODECYLMALTOSIDE, 5 MM MES, PH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 80 (mg/ml) | |
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | 10 (mM) |
