2PPL
Human Pancreatic lipase-related protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.871, 62.871, 306.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.920 - 2.200 |
| R-factor | 0.18268 |
| Rwork | 0.180 |
| R-free | 0.23289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rp1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.218 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 32518 | |
| <I/σ(I)> | 27.6 | 4.4 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 12.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | 20% PEG 4000, 0.2M Ca acetate, 0.1 Na cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K, pH 6.50 |
