2PPI
Structure of the BTB (Tramtrack and Bric a brac) domain of human Gigaxonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 75.664, 75.664, 167.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.900 - 2.400 |
| R-factor | 0.28185 |
| Rwork | 0.281 |
| R-free | 0.29556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1buo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.221 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 | 0.691 |
| Number of reflections | 9876 | |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 5.85 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 282 | 0.1 M NaNO3, 0.1 M Na2SO4, 0.05 M Na/KPO4, 0.0285 M (NH4)2SO4, 30% PEG3350, 10% Ethylene glycol pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 282K |
