2PP6
Crystal structure of the ATP-binding sugar transporter-like protein from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97983 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 51.820, 51.820, 69.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.400 - 2.700 |
| R-factor | 0.216 |
| Rwork | 0.210 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.155 | 0.949 |
| Number of reflections | 2872 | |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 15.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.4 M Ammonium citrate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
