2POS
Crystal Structure of sylvaticin, a new secreted protein from pythium sylvaticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.220, 46.000, 55.730 |
| Unit cell angles | 85.20, 71.27, 74.63 |
Refinement procedure
| Resolution | 21.060 - 1.600 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Sylvaticin C2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.590 |
| Rmerge | 0.048 | 0.120 |
| Number of reflections | 40560 | |
| <I/σ(I)> | 18.1 | 9.22 |
| Completeness [%] | 92.0 | 91.3 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 40% PEG 2000MME, 10mM NiCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
